Archives of Microbiology & Immunology

ISSN: 2572-9365
Impact Factor: 1.1
Index Copernicus Value: 74.70
Abstracting and Indexing

Editor In Chief

Masashi Emoto

Professor of Laboratory of Immunology
Department of Laboratory Sciences
Gunma University Graduate School of Health Sciences
Gunma, Japan

 

Abstract

Comparative Analysis of Quantitative Real-time PCR and Agglutination Detection in Candida Species Isolated from Clinical Urine Specimens of Old Patients

Objective: The study aimed to aim to detect the type of Candida species isolated from the urine samples of older patients. Methods: We collected the urine samples of 102 older patients. We divided the patients into 2 groups (the control group and the experimental group) randomly. The control group used the agglutination detection. The experiment group used the Quantitative Real-time PCR detection. We detected and analysis the data in the groups. Results: The positive ratio of the experimental group was 80.39 % (41/51), on the other side, the positive ratio of the Experiment group was 68.62%(35/51). The positive ratio of the experimental group was higher than the control group (P<0.05). The detect ratio of Candida albicans of the experimental group and control group was 53.5% and 51.4%. Conclusion: Our finding demonstrated that the Quantitative Real-time PCR detection method was more effective than the agglutination detection in the urine sample of older patients.

Author(s):

Yishu Tang, Yang Yang, Ru Wu, Miao Gao, Fang Cui

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